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Sequencing RNA Level 1

Attribute: NGS RNA End Bias

Valid Value Description Ontology
3 Prime An artifact of cDNA amplification and cloning where the 3' end of transcripts is over-represented in the final sample or library.NCIT:C202270
5 Prime An artifact of cDNA amplification and cloning where the 5' end of transcripts is over-represented in the final sample or library.NCIT:C202271
Full Length TranscriptA cDNA molecule that spans the entire length of an RNA transcript, from its 5' end to its 3' end. NCIT:C202272

Attribute: NGS RNA Reverse Transcription Primer

Valid Value Description Ontology
Oligo-dT DNA primers consisting of 12-18 deoxythymidines that anneal to the poly(A) tails of eukaryotic mRNAs and are used to prime reverse transcription. NCIT:C201929
Poly-dT DNA primers consisting of deoxythymidines that anneal to the poly(A) tails of eukaryotic mRNAs and are used to prime reverse transcription. NCIT:C201929
Feature barcodingA technology platform that uses oligonucleotide barcodes to simultaneously measure gene expression and other cellular features (surface proteins, T/B cell receptors, CRISPR perturbations) at single-cell resolution.NCIT:C202255
Random Short oligonucleotides of random sequence that anneal throughout the length of RNA templates and are used to prime reverse transcription. NCIT:C201930