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Visium RNA Level 3

Attribute: Visium File Type

Valid Value Description Ontology
reference png PNG image of the tissue section supplied as the Space Ranger alignment reference image, used to register the capture-area spot grid to the tissue for spatial gene expression analysis. Not available
reference jpg JPEG image of the tissue section supplied as the Space Ranger alignment reference image, used to register the capture-area spot grid to the tissue for spatial gene expression analysis. Not available
json scale factorsJSON file output by Space Ranger (scalefactors_json.json) that records the scaling factors needed to relate spot positions to the full-resolution, high-resolution, and low-resolution tissue images. Not available
probe dataset csv CSV file listing the probe set used in a probe-based (e.g., FFPE) Visium assay, including probe IDs and the genes they target. Not available
qc result html HTML quality-control report summarizing metrics from Space Ranger processing of a Visium sample, such as sequencing, alignment, and spatial coverage statistics. EDAM:data_3914
filtered mex Filtered feature-barcode expression matrix in Market Exchange (MEX) sparse-matrix format, containing only spots that Space Ranger called as being covered by tissue in a Visium sample. NCIT:C184778
unfiltered mex Unfiltered feature-barcode expression matrix in Market Exchange (MEX) sparse-matrix format, containing all spots on the capture area regardless of tissue coverage, output by Space Ranger. NCIT:C184778
tissue_positions Space Ranger output file (tissue_positions.csv/.parquet) that maps each spot barcode to its row/column array coordinates and pixel coordinates on the full-resolution tissue image, and indicates whether the spot overlaps tissue.Not available
barcodes Space Ranger output file (barcodes.tsv) listing the spot barcode sequences corresponding to the columns of the feature-barcode expression matrix. Not available
features Space Ranger output file (features.tsv) listing the gene/feature IDs and names corresponding to the rows of the feature-barcode expression matrix. Not available
fiducial image pngPNG image showing the fiducial frame (alignment markers) printed on the Visium slide, used by Space Ranger/Loupe Browser to register the capture area to the tissue image. Not available
fiducial image jpgJPEG image showing the fiducial frame (alignment markers) printed on the Visium slide, used by Space Ranger/Loupe Browser to register the capture area to the tissue image. Not available
detected image pngPNG image output by Space Ranger showing the tissue area and spots detected during the tissue-detection step, overlaid on the slide image. Not available
detected jpg JPEG image output by Space Ranger showing the tissue area and spots detected during the tissue-detection step, overlaid on the slide image. Not available
high res image High-resolution tissue microscopy image (tissue_hires_image.png) bundled in Space Ranger spatial output, used for detailed visualization of the tissue section with the overlaid spot grid. Not available
low res image Low-resolution, downsampled tissue microscopy image (tissue_lowres_image.png) bundled in Space Ranger spatial output, used for lightweight visualization of the tissue section with the overlaid spot grid. Not available
json scale factorsJSON file output by Space Ranger (scalefactors_json.json) that records the scaling factors needed to relate spot positions to the full-resolution, high-resolution, and low-resolution tissue images. Not available
probe dataset csv CSV file listing the probe set used in a probe-based (e.g., FFPE) Visium assay, including probe IDs and the genes they target. Not available